Journal: Development (Cambridge, England)
Article Title: A Mesp1 -dependent developmental breakpoint in transcriptional and epigenomic specification of early cardiac precursors
doi: 10.1242/dev.201229
Figure Lengend Snippet: Transcriptional profiles of cardiac mesoderm in Mesp1 KO embryos. (A,F,K) scRNA-seq UMAP atlases of Ai14, Smarcd3 -F6 double-positive mesoderm cells from early (A; 5504 cells), middle (F; 7666 cells) and late (K; 22,622 cells) developmental embryo stages. (B,C,G,H,L,M) Associated UMAPs colored by genotype (B,G,L) and Smarcd3 -F6-eGFP expression (C,H,M). (D,E) Early mesoderm DGE in LSMeso2 (D) and EomesPSMeso (E). (I,J) Middle mesoderm DGE in LSMeso2 (I) and Mesp1ME (J). (N-P) Late mesoderm DGE in Meso2 (N), Meso1 (O), and postLPM (P). Significant changes denoted with adjusted P <0.05. antPrSoM, anterior presomitic mesoderm; DE-ME, definitive endoderm/mesendoderm; Foxc2M, Foxc2 + mesoderm; Meso, mesoderm; PSMeso, primitive streak mesoderm; PS-NM, primitive streak/neuromesodermal.
Article Snippet: Antibodies used in this study were: sheep polyclonal Foxc2 (R&D Systems, AF6989), chicken polyclonal GFP (Aves Labs, GFP-1020), rabbit polyclonal Cre (Millipore, 69050).
Techniques: Expressing